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Leinco Technologies 10x flow cytometry stain (fcm) buffer
10x Flow Cytometry Stain (Fcm) Buffer, supplied by Leinco Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/10x+flow+cytometry+stain+(fcm)+buffer/10x+flow+cytometry+stain++fcm++buffer/pmc06788635-327-128-138
Average 90 stars, based on 1 article reviews
10x flow cytometry stain (fcm) buffer - by Bioz Stars, 2026-10
90/100 stars

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Staining:

Article Title: Monitoring of Measurable Residual Disease in Multiple Myeloma by Multiparametric Flow Cytometry
Article Snippet: LIVE/DEAD TM Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific, Catalog # L34957 ); after suspending in DMSO as recommended by the manufacturer, a working stock is prepared by diluting 1:10 in PBS; store at 4 – 8 ̊C for no longer than 24 hours; LIVE/DEAD Fixable Aqua is used as if it was a surface mAb; samples are stained with 5 μL of the working stock 12 × 75 mm Polystyrene Tubes (BD Falcon, Catalog #352052) 10-mL and 50-mL conical centrifuge tubes Ammonium-Chloride (ACK) Lysing Buffer (Thermo Fisher Scientific, Catalog #A1049201); store at room temperature (RT) 10X BD FACS TM Lysing Solution (BD, Catalog #349202); store at RT; dilute in purified water prior to use 10% Methanol-free Formaldehyde, Ultra-Pure (Polysciences, Catalog #04018–1); store at RT; dilute in Phosphate Buffered Saline (PBS) 10X Flow Cytometry Stain (FCM) Buffer (Leinco Technologies, Catalog #F1175) containing 0.5% Bovine Serum Albumin (BSA), 0.1% Sodium Azide (NaN 3 ), and 0.04 g/L disodium ethylenediaminetetraacetic acid (NA 2 EDTA) in PBS; store at 4 o C; dilute 1:10 with deionized water Permeabilization Medium B (Thermo Fisher Scientific, Catalog #GAS002S-100); store at RT; dilute 1:4 with PBS to prepare a working solution Equipment: list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 Clinically-validated flow cytometer equipped with suitable excitation lasers and detectors for measuring fluorescence generated from BV421, BV510, FITC, PE, PCPCy5.5, PECy7, APC, and APCH7 Automated cell counter Centrifuge capable of at least 520 × g Falcon® 70 μm cell strainer (Corning, Catalog #352350) Protocols Steps: list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Filter bone marrow sample through a 70 μm cell strainer to exclude spicules from the sample.

Article Title: Monitoring of Measurable Residual Disease in Multiple Myeloma by Multiparametric Flow Cytometry
Article Snippet: LIVE/DEAD TM Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific, Catalog # L34957 ); after suspending in DMSO as recommended by the manufacturer, a working stock is prepared by diluting 1:10 in PBS; store at 4 – 8 ̊C for no longer than 24 hours; LIVE/DEAD Fixable Aqua is used as if it was a surface mAb; samples are stained with 5 μL of the working stock 12 × 75 mm Polystyrene Tubes (BD Falcon, Catalog #352052) 10-mL and 50-mL conical centrifuge tubes Ammonium-Chloride (ACK) Lysing Buffer (Thermo Fisher Scientific, Catalog #A1049201); store at room temperature (RT) 10X BD FACS TM Lysing Solution (BD, Catalog #349202); store at RT; dilute in purified water prior to use 10% Methanol-free Formaldehyde, Ultra-Pure (Polysciences, Catalog #04018–1); store at RT; dilute in Phosphate Buffered Saline (PBS) 10X Flow Cytometry Stain (FCM) Buffer (Leinco Technologies, Catalog #F1175) containing 0.5% Bovine Serum Albumin (BSA), 0.1% Sodium Azide (NaN 3 ), and 0.04 g/L disodium ethylenediaminetetraacetic acid (NA 2 EDTA) in PBS; store at 4 o C; dilute 1:10 with deionized water Permeabilization Medium B (Thermo Fisher Scientific, Catalog #GAS002S-100); store at RT; dilute 1:4 with PBS to prepare a working solution

FACS:

Article Title: Monitoring of Measurable Residual Disease in Multiple Myeloma by Multiparametric Flow Cytometry
Article Snippet: LIVE/DEAD TM Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific, Catalog # L34957 ); after suspending in DMSO as recommended by the manufacturer, a working stock is prepared by diluting 1:10 in PBS; store at 4 – 8 ̊C for no longer than 24 hours; LIVE/DEAD Fixable Aqua is used as if it was a surface mAb; samples are stained with 5 μL of the working stock 12 × 75 mm Polystyrene Tubes (BD Falcon, Catalog #352052) 10-mL and 50-mL conical centrifuge tubes Ammonium-Chloride (ACK) Lysing Buffer (Thermo Fisher Scientific, Catalog #A1049201); store at room temperature (RT) 10X BD FACS TM Lysing Solution (BD, Catalog #349202); store at RT; dilute in purified water prior to use 10% Methanol-free Formaldehyde, Ultra-Pure (Polysciences, Catalog #04018–1); store at RT; dilute in Phosphate Buffered Saline (PBS) 10X Flow Cytometry Stain (FCM) Buffer (Leinco Technologies, Catalog #F1175) containing 0.5% Bovine Serum Albumin (BSA), 0.1% Sodium Azide (NaN 3 ), and 0.04 g/L disodium ethylenediaminetetraacetic acid (NA 2 EDTA) in PBS; store at 4 o C; dilute 1:10 with deionized water Permeabilization Medium B (Thermo Fisher Scientific, Catalog #GAS002S-100); store at RT; dilute 1:4 with PBS to prepare a working solution Equipment: list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 Clinically-validated flow cytometer equipped with suitable excitation lasers and detectors for measuring fluorescence generated from BV421, BV510, FITC, PE, PCPCy5.5, PECy7, APC, and APCH7 Automated cell counter Centrifuge capable of at least 520 × g Falcon® 70 μm cell strainer (Corning, Catalog #352350) Protocols Steps: list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Filter bone marrow sample through a 70 μm cell strainer to exclude spicules from the sample.

Article Title: Monitoring of Measurable Residual Disease in Multiple Myeloma by Multiparametric Flow Cytometry
Article Snippet: LIVE/DEAD TM Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific, Catalog # L34957 ); after suspending in DMSO as recommended by the manufacturer, a working stock is prepared by diluting 1:10 in PBS; store at 4 – 8 ̊C for no longer than 24 hours; LIVE/DEAD Fixable Aqua is used as if it was a surface mAb; samples are stained with 5 μL of the working stock 12 × 75 mm Polystyrene Tubes (BD Falcon, Catalog #352052) 10-mL and 50-mL conical centrifuge tubes Ammonium-Chloride (ACK) Lysing Buffer (Thermo Fisher Scientific, Catalog #A1049201); store at room temperature (RT) 10X BD FACS TM Lysing Solution (BD, Catalog #349202); store at RT; dilute in purified water prior to use 10% Methanol-free Formaldehyde, Ultra-Pure (Polysciences, Catalog #04018–1); store at RT; dilute in Phosphate Buffered Saline (PBS) 10X Flow Cytometry Stain (FCM) Buffer (Leinco Technologies, Catalog #F1175) containing 0.5% Bovine Serum Albumin (BSA), 0.1% Sodium Azide (NaN 3 ), and 0.04 g/L disodium ethylenediaminetetraacetic acid (NA 2 EDTA) in PBS; store at 4 o C; dilute 1:10 with deionized water Permeabilization Medium B (Thermo Fisher Scientific, Catalog #GAS002S-100); store at RT; dilute 1:4 with PBS to prepare a working solution

Purification:

Article Title: Monitoring of Measurable Residual Disease in Multiple Myeloma by Multiparametric Flow Cytometry
Article Snippet: LIVE/DEAD TM Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific, Catalog # L34957 ); after suspending in DMSO as recommended by the manufacturer, a working stock is prepared by diluting 1:10 in PBS; store at 4 – 8 ̊C for no longer than 24 hours; LIVE/DEAD Fixable Aqua is used as if it was a surface mAb; samples are stained with 5 μL of the working stock 12 × 75 mm Polystyrene Tubes (BD Falcon, Catalog #352052) 10-mL and 50-mL conical centrifuge tubes Ammonium-Chloride (ACK) Lysing Buffer (Thermo Fisher Scientific, Catalog #A1049201); store at room temperature (RT) 10X BD FACS TM Lysing Solution (BD, Catalog #349202); store at RT; dilute in purified water prior to use 10% Methanol-free Formaldehyde, Ultra-Pure (Polysciences, Catalog #04018–1); store at RT; dilute in Phosphate Buffered Saline (PBS) 10X Flow Cytometry Stain (FCM) Buffer (Leinco Technologies, Catalog #F1175) containing 0.5% Bovine Serum Albumin (BSA), 0.1% Sodium Azide (NaN 3 ), and 0.04 g/L disodium ethylenediaminetetraacetic acid (NA 2 EDTA) in PBS; store at 4 o C; dilute 1:10 with deionized water Permeabilization Medium B (Thermo Fisher Scientific, Catalog #GAS002S-100); store at RT; dilute 1:4 with PBS to prepare a working solution Equipment: list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 Clinically-validated flow cytometer equipped with suitable excitation lasers and detectors for measuring fluorescence generated from BV421, BV510, FITC, PE, PCPCy5.5, PECy7, APC, and APCH7 Automated cell counter Centrifuge capable of at least 520 × g Falcon® 70 μm cell strainer (Corning, Catalog #352350) Protocols Steps: list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Filter bone marrow sample through a 70 μm cell strainer to exclude spicules from the sample.

Article Title: Monitoring of Measurable Residual Disease in Multiple Myeloma by Multiparametric Flow Cytometry
Article Snippet: LIVE/DEAD TM Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific, Catalog # L34957 ); after suspending in DMSO as recommended by the manufacturer, a working stock is prepared by diluting 1:10 in PBS; store at 4 – 8 ̊C for no longer than 24 hours; LIVE/DEAD Fixable Aqua is used as if it was a surface mAb; samples are stained with 5 μL of the working stock 12 × 75 mm Polystyrene Tubes (BD Falcon, Catalog #352052) 10-mL and 50-mL conical centrifuge tubes Ammonium-Chloride (ACK) Lysing Buffer (Thermo Fisher Scientific, Catalog #A1049201); store at room temperature (RT) 10X BD FACS TM Lysing Solution (BD, Catalog #349202); store at RT; dilute in purified water prior to use 10% Methanol-free Formaldehyde, Ultra-Pure (Polysciences, Catalog #04018–1); store at RT; dilute in Phosphate Buffered Saline (PBS) 10X Flow Cytometry Stain (FCM) Buffer (Leinco Technologies, Catalog #F1175) containing 0.5% Bovine Serum Albumin (BSA), 0.1% Sodium Azide (NaN 3 ), and 0.04 g/L disodium ethylenediaminetetraacetic acid (NA 2 EDTA) in PBS; store at 4 o C; dilute 1:10 with deionized water Permeabilization Medium B (Thermo Fisher Scientific, Catalog #GAS002S-100); store at RT; dilute 1:4 with PBS to prepare a working solution

Flow Cytometry:

Article Title: Monitoring of Measurable Residual Disease in Multiple Myeloma by Multiparametric Flow Cytometry
Article Snippet: LIVE/DEAD TM Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific, Catalog # L34957 ); after suspending in DMSO as recommended by the manufacturer, a working stock is prepared by diluting 1:10 in PBS; store at 4 – 8 ̊C for no longer than 24 hours; LIVE/DEAD Fixable Aqua is used as if it was a surface mAb; samples are stained with 5 μL of the working stock 12 × 75 mm Polystyrene Tubes (BD Falcon, Catalog #352052) 10-mL and 50-mL conical centrifuge tubes Ammonium-Chloride (ACK) Lysing Buffer (Thermo Fisher Scientific, Catalog #A1049201); store at room temperature (RT) 10X BD FACS TM Lysing Solution (BD, Catalog #349202); store at RT; dilute in purified water prior to use 10% Methanol-free Formaldehyde, Ultra-Pure (Polysciences, Catalog #04018–1); store at RT; dilute in Phosphate Buffered Saline (PBS) 10X Flow Cytometry Stain (FCM) Buffer (Leinco Technologies, Catalog #F1175) containing 0.5% Bovine Serum Albumin (BSA), 0.1% Sodium Azide (NaN 3 ), and 0.04 g/L disodium ethylenediaminetetraacetic acid (NA 2 EDTA) in PBS; store at 4 o C; dilute 1:10 with deionized water Permeabilization Medium B (Thermo Fisher Scientific, Catalog #GAS002S-100); store at RT; dilute 1:4 with PBS to prepare a working solution Equipment: list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 Clinically-validated flow cytometer equipped with suitable excitation lasers and detectors for measuring fluorescence generated from BV421, BV510, FITC, PE, PCPCy5.5, PECy7, APC, and APCH7 Automated cell counter Centrifuge capable of at least 520 × g Falcon® 70 μm cell strainer (Corning, Catalog #352350) Protocols Steps: list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Filter bone marrow sample through a 70 μm cell strainer to exclude spicules from the sample.

Article Title: Monitoring of Measurable Residual Disease in Multiple Myeloma by Multiparametric Flow Cytometry
Article Snippet: LIVE/DEAD TM Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific, Catalog # L34957 ); after suspending in DMSO as recommended by the manufacturer, a working stock is prepared by diluting 1:10 in PBS; store at 4 – 8 ̊C for no longer than 24 hours; LIVE/DEAD Fixable Aqua is used as if it was a surface mAb; samples are stained with 5 μL of the working stock 12 × 75 mm Polystyrene Tubes (BD Falcon, Catalog #352052) 10-mL and 50-mL conical centrifuge tubes Ammonium-Chloride (ACK) Lysing Buffer (Thermo Fisher Scientific, Catalog #A1049201); store at room temperature (RT) 10X BD FACS TM Lysing Solution (BD, Catalog #349202); store at RT; dilute in purified water prior to use 10% Methanol-free Formaldehyde, Ultra-Pure (Polysciences, Catalog #04018–1); store at RT; dilute in Phosphate Buffered Saline (PBS) 10X Flow Cytometry Stain (FCM) Buffer (Leinco Technologies, Catalog #F1175) containing 0.5% Bovine Serum Albumin (BSA), 0.1% Sodium Azide (NaN 3 ), and 0.04 g/L disodium ethylenediaminetetraacetic acid (NA 2 EDTA) in PBS; store at 4 o C; dilute 1:10 with deionized water Permeabilization Medium B (Thermo Fisher Scientific, Catalog #GAS002S-100); store at RT; dilute 1:4 with PBS to prepare a working solution

Fluorescence:

Article Title: Monitoring of Measurable Residual Disease in Multiple Myeloma by Multiparametric Flow Cytometry
Article Snippet: LIVE/DEAD TM Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific, Catalog # L34957 ); after suspending in DMSO as recommended by the manufacturer, a working stock is prepared by diluting 1:10 in PBS; store at 4 – 8 ̊C for no longer than 24 hours; LIVE/DEAD Fixable Aqua is used as if it was a surface mAb; samples are stained with 5 μL of the working stock 12 × 75 mm Polystyrene Tubes (BD Falcon, Catalog #352052) 10-mL and 50-mL conical centrifuge tubes Ammonium-Chloride (ACK) Lysing Buffer (Thermo Fisher Scientific, Catalog #A1049201); store at room temperature (RT) 10X BD FACS TM Lysing Solution (BD, Catalog #349202); store at RT; dilute in purified water prior to use 10% Methanol-free Formaldehyde, Ultra-Pure (Polysciences, Catalog #04018–1); store at RT; dilute in Phosphate Buffered Saline (PBS) 10X Flow Cytometry Stain (FCM) Buffer (Leinco Technologies, Catalog #F1175) containing 0.5% Bovine Serum Albumin (BSA), 0.1% Sodium Azide (NaN 3 ), and 0.04 g/L disodium ethylenediaminetetraacetic acid (NA 2 EDTA) in PBS; store at 4 o C; dilute 1:10 with deionized water Permeabilization Medium B (Thermo Fisher Scientific, Catalog #GAS002S-100); store at RT; dilute 1:4 with PBS to prepare a working solution Equipment: list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 Clinically-validated flow cytometer equipped with suitable excitation lasers and detectors for measuring fluorescence generated from BV421, BV510, FITC, PE, PCPCy5.5, PECy7, APC, and APCH7 Automated cell counter Centrifuge capable of at least 520 × g Falcon® 70 μm cell strainer (Corning, Catalog #352350) Protocols Steps: list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Filter bone marrow sample through a 70 μm cell strainer to exclude spicules from the sample.

Article Title: Monitoring of Measurable Residual Disease in Multiple Myeloma by Multiparametric Flow Cytometry
Article Snippet: LIVE/DEAD TM Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific, Catalog # L34957 ); after suspending in DMSO as recommended by the manufacturer, a working stock is prepared by diluting 1:10 in PBS; store at 4 – 8 ̊C for no longer than 24 hours; LIVE/DEAD Fixable Aqua is used as if it was a surface mAb; samples are stained with 5 μL of the working stock 12 × 75 mm Polystyrene Tubes (BD Falcon, Catalog #352052) 10-mL and 50-mL conical centrifuge tubes Ammonium-Chloride (ACK) Lysing Buffer (Thermo Fisher Scientific, Catalog #A1049201); store at room temperature (RT) 10X BD FACS TM Lysing Solution (BD, Catalog #349202); store at RT; dilute in purified water prior to use 10% Methanol-free Formaldehyde, Ultra-Pure (Polysciences, Catalog #04018–1); store at RT; dilute in Phosphate Buffered Saline (PBS) 10X Flow Cytometry Stain (FCM) Buffer (Leinco Technologies, Catalog #F1175) containing 0.5% Bovine Serum Albumin (BSA), 0.1% Sodium Azide (NaN 3 ), and 0.04 g/L disodium ethylenediaminetetraacetic acid (NA 2 EDTA) in PBS; store at 4 o C; dilute 1:10 with deionized water Permeabilization Medium B (Thermo Fisher Scientific, Catalog #GAS002S-100); store at RT; dilute 1:4 with PBS to prepare a working solution

Generated:

Article Title: Monitoring of Measurable Residual Disease in Multiple Myeloma by Multiparametric Flow Cytometry
Article Snippet: LIVE/DEAD TM Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific, Catalog # L34957 ); after suspending in DMSO as recommended by the manufacturer, a working stock is prepared by diluting 1:10 in PBS; store at 4 – 8 ̊C for no longer than 24 hours; LIVE/DEAD Fixable Aqua is used as if it was a surface mAb; samples are stained with 5 μL of the working stock 12 × 75 mm Polystyrene Tubes (BD Falcon, Catalog #352052) 10-mL and 50-mL conical centrifuge tubes Ammonium-Chloride (ACK) Lysing Buffer (Thermo Fisher Scientific, Catalog #A1049201); store at room temperature (RT) 10X BD FACS TM Lysing Solution (BD, Catalog #349202); store at RT; dilute in purified water prior to use 10% Methanol-free Formaldehyde, Ultra-Pure (Polysciences, Catalog #04018–1); store at RT; dilute in Phosphate Buffered Saline (PBS) 10X Flow Cytometry Stain (FCM) Buffer (Leinco Technologies, Catalog #F1175) containing 0.5% Bovine Serum Albumin (BSA), 0.1% Sodium Azide (NaN 3 ), and 0.04 g/L disodium ethylenediaminetetraacetic acid (NA 2 EDTA) in PBS; store at 4 o C; dilute 1:10 with deionized water Permeabilization Medium B (Thermo Fisher Scientific, Catalog #GAS002S-100); store at RT; dilute 1:4 with PBS to prepare a working solution Equipment: list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 Clinically-validated flow cytometer equipped with suitable excitation lasers and detectors for measuring fluorescence generated from BV421, BV510, FITC, PE, PCPCy5.5, PECy7, APC, and APCH7 Automated cell counter Centrifuge capable of at least 520 × g Falcon® 70 μm cell strainer (Corning, Catalog #352350) Protocols Steps: list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Filter bone marrow sample through a 70 μm cell strainer to exclude spicules from the sample.

Article Title: Monitoring of Measurable Residual Disease in Multiple Myeloma by Multiparametric Flow Cytometry
Article Snippet: LIVE/DEAD TM Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific, Catalog # L34957 ); after suspending in DMSO as recommended by the manufacturer, a working stock is prepared by diluting 1:10 in PBS; store at 4 – 8 ̊C for no longer than 24 hours; LIVE/DEAD Fixable Aqua is used as if it was a surface mAb; samples are stained with 5 μL of the working stock 12 × 75 mm Polystyrene Tubes (BD Falcon, Catalog #352052) 10-mL and 50-mL conical centrifuge tubes Ammonium-Chloride (ACK) Lysing Buffer (Thermo Fisher Scientific, Catalog #A1049201); store at room temperature (RT) 10X BD FACS TM Lysing Solution (BD, Catalog #349202); store at RT; dilute in purified water prior to use 10% Methanol-free Formaldehyde, Ultra-Pure (Polysciences, Catalog #04018–1); store at RT; dilute in Phosphate Buffered Saline (PBS) 10X Flow Cytometry Stain (FCM) Buffer (Leinco Technologies, Catalog #F1175) containing 0.5% Bovine Serum Albumin (BSA), 0.1% Sodium Azide (NaN 3 ), and 0.04 g/L disodium ethylenediaminetetraacetic acid (NA 2 EDTA) in PBS; store at 4 o C; dilute 1:10 with deionized water Permeabilization Medium B (Thermo Fisher Scientific, Catalog #GAS002S-100); store at RT; dilute 1:4 with PBS to prepare a working solution



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Leinco Technologies 10x flow cytometry stain (fcm) buffer
10x Flow Cytometry Stain (Fcm) Buffer, supplied by Leinco Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/10x+flow+cytometry+stain+(fcm)+buffer/10x+flow+cytometry+stain++fcm++buffer/pmc06788635-327-128-138
Average 90 stars, based on 1 article reviews
10x flow cytometry stain (fcm) buffer - by Bioz Stars, 2026-10
90/100 stars
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